While a gene copy number (CN) can be estimated by NGS, your may require ISH, still a “gold standard,” to assess a target gene amplification or loss. The test can be performed on smaller amounts of tissue compared to NGS. ISH offering includes the assays approved by FDA as companion diagnostics or patient management.

Acutis offers qPCR as an alternative to NGS in some cases where the target can be limited to specific mutations that can be performed on smaller amount of sample with a quicker turn-around-time. Our PCR offering includes the assays approved by FDA as companion diagnostics or patient management.

ddPCR is a novel molecular biology technique that provides absolute quantification of target nucleic acids without the need for an external calibrator. While qPCR assay has a broader dynamic range, ddPCR assay has a significantly higher degree of sensitivity and precision.

A tissue sample can be profiled at nucleic acid level via NGS, PCR, ddPCR, or ISH but IHC is unique in its ability to characterize a biomarker at a protein level and can demonstrate the protein spatial configuration within tumor cells or tumor microenvironment. The test can be performed on a tiny amount of tissue within a short turn-around-time. IHC offering includes the assays approved by FDA as companion diagnostics or patient management.

In addition to screening of each ISH or IHC slides by a board-certified pathologist, Acutis performs full slide scan to score broader areas of interests and provide the scan to a client for independent review, making annotated image for a presentation or publication, and/or use the scan as a permanent study record.